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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

in Papers i owe my pipettes by Priya Nair

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity on “Papers i owe my pipettes”, a list by Priya Nair on TheLysts.

Details

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Name
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Field
CRISPR mechanism
What it’s about
Jinek et al. showing Cas9 can be steered by dual RNAs to cut DNA in bacteria, basically revealing the core machinery we all now abuse for editing.
My take
the primordial soup of my day job. every time i design a guide, this is the ghost in the machine.